Miljø- og Fødevareudvalget 2015-16
MOF Alm.del Bilag 660
Offentligt
1669735_0001.png
fn
g
ila
in, BlaZkova 69501Hodonin,cZ. TestingLaboratoryNo.1273certifiedby czechAccreditation
5.
ax+420518340919
#a.
CopyNo.:I
Issue
No.: 1
DETERMINATION OF BACTERICIDAL AND FIINGICIDAL ACTIVITY
OF THE PRODUCT
DETOXY COLOR ON CARRIERS
Sample : 209 -209 I 2012
ID
4
5
Samplename:
DETOXY COLOR
Clienf ROKOSPOL
a.s.,Krakovsk| t346ll1,praha 1
Producer:
RoKosPoL a.s.,
provozKaf,ovice, 4r Biskupice Luhadovice
763
u
samplingpoint RoKoSPoL a.s.,
provozKariovice,
763 4l Biskupiceu Lrfiadovice
Page: 1
From pages: 8
Incoming date:
20.9.2012
Delivery date:
24.r.2013
Hodonin,
24.1.2013
ChenQila, upor. r.o.
r
The report may be reproduced only as a whole, in parls only upon written permission
ofthe laboratory. The test results relate only to the
sarnples stated in the Test Report. The Lab does not take any guarantee for
the identity of sampies not taken by the lab personnel.
 MOF, Alm.del - 2015-16 - Bilag 660: Henvendelse af 27/9-16 fra Kenneth Riis, Lifeguarders, om MRSA - Gør man overhovedet noget ved problemet
1669735_0002.png
Description: Testing the fficacy of chemical disinfectants and antiseptics
Sample : 209 -209 I 2012
ID
4
5
RepNo: 111
Sample
name:DETOXY COLOR
Sampled: client
by
point ROKOSPOLa.s.,Biskupiceu Lrhadovice
Sampling
praha1
Client ROKOSPOL
a.s.,Krakovsk| 1346115.
Samplingdate:20.9.2012
Sample
delivered:
20.9.2012
Testingdate: 10. 10.12.2012
22. -
Delivered
amount: kg + 0.5kg
0.5
BatchNo: 2700410,
laboratory
sample
Page:2
Subject
oftestin$
Determination bactericidal fungicidalactivityof theproducton carriers.
of
and
Identification the sample:
of
Sample
ID:
Nameof theproduct:
Batchnumber:
Dateof manufacture:
Expiry date:
Manufacturer:
Incomingdate:
Storage
conditions:
209412012
DETOXY COLOR (paintwith nanoTiOz)
27004t0
13.8.2012
Oneyearin a perfectlyclosed
containers temperatures +5 to +25 o C
at
from
ROKOSPOLa.s.,provozKariovice,763 4lBiskupice u Luhadovice
20.9.20t2
+5 - +25 0C
nano TiOr
Active compounds and concentrations:
Sample
ID:
Nameof theproduct:
Batchnumber:
Dateof manufacture:
Expiry date:
Manufacturer:
Incomingdate:
Storage
conditions:
2094/2012
DETOXY COLOR (paintwithoutnanoTiOz)
laboratory
sample
r6.8.2012
not available
ROKOSPOL
a.s.,provozKariovice,
763 4lBiskupice u Lrhadovice
20.9.2012
+5 - +25 0C
without active compounds
Active compounds and concentrations:
.t"
 MOF, Alm.del - 2015-16 - Bilag 660: Henvendelse af 27/9-16 fra Kenneth Riis, Lifeguarders, om MRSA - Gør man overhovedet noget ved problemet
1669735_0003.png
Description: Testing the fficacy of chemical disinfectantsand antiseptics
Sample ID:2094-209512012
Rep No: 111
Samplename: DETOXY COLOR
Sampled: by client
Sampling point ROKOSPOL a.s.,Biskupice u Lrhadovice
Client: ROKOSPOL a.s.. Krakovsk| 1346115"
Praha 1
Sampling date:20.9.2012
Sampledelivered:209.2012
Testing date:22.10.-10.12.2012
Delivered amount: 0.5 kg + 0.5 kg
Batch No: 27004l0,laboratory sample
Paee: 3
Experimental conditions:
Period of analysis:
Test temperature:
Test method:
Neutralization medium:
Appearanceof the products:
Test concentration:
Contact time:
Interfering substances:
Test organisms:
Incubation conditions:
Quantitative test on carriers for the evaluation of bactericidal
activity SOP-M-19-00-C(CSN EN 13697)
22. I0. - 23. 10.2012 pant application
22. 10. - 6. ll. 2012 conditioning of the coating on carriers at
temperature
23.5'C + 1.9 oC and humidity 58.4 "H + 4.7 oH
6. 11. * 21. 11,.2012 test on carriers
23 "C + 2 "C
dilution neutralization method
Dey-EngleyNeutralizrngBroth M 1062
white liquid
100% (concentrated,application of the product on carriers - paint,
12 hours drytng, new paint, another 12 hours dryrng)
2 hours, 24 hours, I week and 2 weeks
according to the customer'srequestwithout interfering substances
Pseudomonasaeruginosa
ATCC 15442
oC. 24 hours
37 "C+ I
Test procedure:
1. Preparation ofthe test suspension
2. Preparation ofproduct test solutions
3. Carrier surfacetreatrnent - paint, 12 hours dryrng, new paint, another 12 hours dryng
4. Quantitative carcier test (carriers for testing were used as required in the standard, first part of carriers
was used without treatment, according to the customer'srequest the second part of carriers was painted
with colour with the active substance*and the third part of carriers the paint without the active substance,
After that the microorganisms were applied on carriers, which were maintained in the constant
environment at temperature 23.3 oC +. 0.2 "C and humidity 40.8 oH + 5.4 oH and under the light with
intensity 841 +34 kn). The density of microorganisms was monitored after 2hours,24 hours, 1 week and
2 weeks.
5. Incubation and calculation
6. Expression and interpretation ofresults
Note:
Bactericidal activity - the capability of a product to produce a reduction in the number of viable bacterial cells of
relevant organismson carriers under defined conditions by at least 4 orders (104).Orying of the suspensionwith
microorganisms on the carrier without paint: 30 - 35 min. Drying of the suspensionwith microorganisms on the
carrier with painl 24 hours.
x The test was modified according to the granted testing scopeflexibility type I.
The standard:
eSN BN 13697 Chemical disinfectants and antiseptiis - Quantitative non-porous surface test for the evaluation
of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and
institutional areas- Test method and requirementswithout mechanical action (phase 2lsrep2)March 2002
R-09-06 Flexible scopeof accreditation in laboratory Chemila, Ltd.
 MOF, Alm.del - 2015-16 - Bilag 660: Henvendelse af 27/9-16 fra Kenneth Riis, Lifeguarders, om MRSA - Gør man overhovedet noget ved problemet
1669735_0004.png
Description: Testing the effrcacy of chemical disinfectantsand antiseptics
Sample 209 -209 I 2012
ID:
4
5
Rep
No: 111
Sample
name:
DETOXY COLOR
Sampled: client
by
Sampling
point ROKOSPOL Biskupice Luhadovice
a.s.,
u
prahaI
Clienf ROKOSPOL
a.s.,Krakovsk| 1346115.
Samplingdate:209.2012
Sample
delivered:
20.9.2012
Testingdate:
22.10.-10.12.2012
Delivered
amount: kg + Q.Jftg
0.5
BatchNo: 2700410,
laboratory
sample
Page:4
c.1 Testingthe efficacyof chemical
disinfectant
DEToxy col,oR on carriers
TabNo. 1.1T
est
Time
bactericidal aktrvrtv of the
Test organism
Bacterial test
suspensionN
DETOXY COLOR
Water control Nc
cariers without
paint
Water control Nc
caniers with paint
withoutTiO2
Test procedure Nd
carriers with paint
with TiO2
0
Pseudomonas
aerugmos4
ATCC 15442
>300
10-">300,
:
l 0 - 14 8 , 5 6
:
N: 7.41
Pseudomonas
aeruginosa
2 hours
A'tcc 15442
Pseudomonas
aeruginosa
ATCC 15442
24 hows
>300
10-': >300,
>300,
>300
10-a:
1 0 - s3 8 ,3 9
:
106:4,4
Nc: 7.59
>300
t0-' : >300,
>300,
>300
t0'a:
l0-5:31,33
lO6:2,4
,,lc 7.51
:
1 0 -
: >300,>300
l0-4
84,80
10-5
R O
10-6
0 , 2
N c :
6.91
I week
Pseudomonas
aerugrnosa
ATCC 15442
Pseudomonas
aerugtnosa
ATCC 15442
1 0 "0 , 0
:
1 0 - '0 , 0
:
l0-20,0
:
10' 0 , 0
10-t
0 , 0
10'2
0 , 0
>300
10-': >300,
>300,
>300
10-a:
>300,
>300
10-5:
10'6:33,32
Nc:8.51
t0-' : >300,>300
>300,
>300
t0-a:
L 0 - 5 :5 1 1 5 7
1
,
t0-6:15,16
t l c :8 . 1 9
1 0 "0 , 0
:
l 0 - t :0 , 0
l0-2 0, 0
:
>300,
>300
10-":
>300,
>300
10-5:
1 0 - 6 :3 , 3 0
3
Nd: 8.50
ME:0
l0':0,0
10't 0,0
:
1 0 - 2 : ,0
0
Nd:< 0.10
ME : > 6.81
1 0 "0 , 0
10-t
0 , 0
10t
0 , 0
10": ,0
0
1 0 -:t 0 , 0
10-20, 0
:
2 weeks
1 0 '0 , 0
:
1 0 - t :, 0
0
10-2: 0
0,
.
N: log1e
[{0.05 (x + x')} 12. dl
where x and x'are paired values for which the mean of the value falls between
50
and 300 colonies, is the
d
dilution factor for the dilution taken into account
N C o r N T : l o g t o [ { 1 0 .( y + y ' ) } 1 2 . d l
where y and y' are paired values for which the mean of the value falls between
50 and 300 colonies, is the
d
dilution factor for the dilution taken into account
 MOF, Alm.del - 2015-16 - Bilag 660: Henvendelse af 27/9-16 fra Kenneth Riis, Lifeguarders, om MRSA - Gør man overhovedet noget ved problemet
1669735_0005.png
Description: Testing the fficacy of chemical disinfectantsand antiseptics
Sample lD:2094-209512012
Rep No: 111
Samplename: DETOXY COLOR
Sampled: by client
Sampling poinf ROKOSPOL a.s.,Biskupice u L*radovice
Client ROKOSPOL a.s.,Krakovsk| I346/75,Praha I
Sampling date:20.9.2012
Sampledelivered:20.9.201.2
Testing date:22.10.-10.12.2012
Delivered amount: 0.5 kg + 0.5 kg
Batch No: 2700410,laboratory sample
Page:5
Experimental conditions:
Period of analysis:
Test temperature:
Test method:
Neutralization medium:
Appearanceof the products:
Test concentration:
Contact time:
Interfering substances:
Test organisms:
Incubation conditions:
Quantitative test on carriers for the evaluation of fungicidal
activity soP-M-19-00-c (esN EN 13697)
22.10. - 23. l0.2012paint application
22. 10. - 6. 11.201,2 conditioning of the coating on carriers at
23.5 oC + 1.9 'C and humidity 58.4 "H + 4.7 oH
temperature
6. lL - 21. ll. 2012 |est on carriers
23 oC + 2 oC
dilution neutralization method
Dey-Engley Neutralizing Broth M 1062
white liquid
100% (concentrated,application of the product on carriers - paint,
12 hours dryttrg, new paint, another 12 hours dryng)
2 hours, 24 hours, I week, 2 weeks, 1 month
according to the customer'srequestwithout interfering substances
ATCC 16 404
Aspergillus niger
oC, 48 hours and additional period of24 or 48 hours
30 "C + 1
Test procedure:
1. Preparationoftest suspension
2. Preparationofproduct test solutions
3. Carriers surfacetreatment - paint, color drying 12 hours, new paint, color drying 12 hours
4. Quantitative carrier test (carriers for testing were used as required in the standard, first part of carriers
was used without treatment, according to the customer'srequest the second part of carriers was painted
with colour with the active substance*and the third part of carriers the paint without the active substance,
After that the microorganisms were applied on carriers, which were maintained in the constant
environment at temperature 233 oC + 0.2 oC and humidity 40.8 "H + 5.4 oH and under the light with
intensity 841 + 34 lm). The density of microorganisms was monitored after 2 hours, 24 hours, I week,2
weeks and 1 month.
5. Incubation and calculation
6. Expression and interpretation ofresults
Note:
Fungicidal actlity - the capability of a product to produce a reduction in the number of viable fungi belonging
to reference strains on carriers under def,rned
conditions by at least 3 orders (103). Drying ofthe suspensionwith
microorganisms on the carrier without paint: 30 - 35 min. Drytng of the suspensionwith microorganisms on the
carrier with painf 24 hours.
* The test was modified according to the granted testing scopeflexibility type I.
The standard:
eSN BN 13697 Chemical disinfectants and antiseptics- Quantitative non-porous surface test for the evaluation
of bactericidal and/or fungicidal activity of chemical disinfectants used in fcibd, industrial, domestic and
institutional areas- Test method and requirementswithout mechanical action (phase 2lstep 2)March2002
R-09-06 Flexible scopeof accreditation in laboratory Chemila, Ltd.
 MOF, Alm.del - 2015-16 - Bilag 660: Henvendelse af 27/9-16 fra Kenneth Riis, Lifeguarders, om MRSA - Gør man overhovedet noget ved problemet
1669735_0006.png
Description: Testing the fficacy of chemical disinfectantsand antiseptics
Sample : 209 -209 / 20t2
ID
4
5
Rep
No: 111
Sample
name:DETOXY COLOR
Sampled: client
by
Sampling
point ROKOSPOL Biskupice Luhadovice
a.s.,
u
Clienf ROKOSPOL Krakovsk|l346ll5.praha1
a.s.,
Samplingdate:20.9.20
12
Sample
delivered:
20.9.2012
Testing
date:
22.10.-10.12.2012
Delivered
amount: kg + Q.J1g
0.5
BatchNo: 2700410,
laboratory
sample
Pase:
6
c.2 Testingthe efficacyof chemical
disinfectant
DEToxy col,oR on carriers
b No.2.1T
o.
Time
cldal akt
vrtv of the
Test organisrr
Fungicidal test
suspensionN
lo-t .)1\
)a1
DETOXY COLOR
Water control Nc
carriers without
paint
Water control Nc
carriers with paint
without TiO2
Test procedure Nd
carriers with paint
with TiO2
Aspergillus niger
ATCC 16 404
0
Aspergillus niger
ATCC 16404
l0-r:24,23
N : 6.08
2 hours
Aspergillus niger
ATCC 16 404
24 hours
Aspergillus niger
ATCC 16 404
I week
Aspergillus niger
ATCC 16404
2 weeks
Aspergillus niger
ATCC 16 404
I rnonth
.
N: logle
[{0.05 (x + x')} /2 . dl
>300,
>300
10-':
>300,
>300
10-r:
1 0 - a :1 , 4 8
5
l0-s: 5
4,
Nc: 6.69
>300
l0-':>300,
>300,
>300
l0-3:
l\-a:41,45
lO's:4,2
\,lc: 6.63
>300
t0-: >300,
t 0 - 3 :0 8 , 2 9 0
3
l 0 - a3 0 , 2 8
:
l0'5:2,2
tlc : 6.48
>300,
>300
10-':
>300,
>300
10-3:
104: 43,
47
10-5: 5
5,
Nc : 6.65
>300,
>300
10-':
>300,
>300
10-3:
10-a: 3 I
35,
1 0s : 2 , 3
Nc: 6.52
>300
l0'': >300,
l0'3:213,230
1 04 : 2 2 , 2 0
l0-5:0,2
Nc: 6.35
>300,
>300
10-':
l0'3:282,269
10'a:27,28
l 0 - s3 , 2
:
Nc: 6.44
>300,
>300
10-':
>300,
>300
10J:
104:32,34
l 0 - 53 , 3
:
Nc:6.52
>300,
>300
t0-":
>300,
>300
L0-3:
l\a:45,40
t0-s: 5
4,
tlc : 6.63
>300
0'': >300,
.0-3:235,247
0-a:24,22
0-s:2,2
,trc 6.38
:
0-':>300,>300
0'3 142,152
0 a :1 4 , 6
1
0-s:2,2
'lc: 6.17
>300,
>300
10-':
>300,
>300
10-r:
10-a:31,30
Nd: 6.48
ME:0.15
>300,
>300
10-':
,10'3:27,29
l } ' a: 3 , 3
Nd: 5.45
ME:1.03
10-':13,15
10-2:2,0
1 0 - 3 :, 0
0
Nd:3.15
ME:3.50
1 0 - : 1 6 ,1 5
'
l0'2:2,2
10-3:0,0
Nd:3.19
ME :3.34
l 0 - ': 6 , 5
10'2:0,2
l 0 - 30 , 0
:
Nd: 2.74
ME:3.61
* and x' are paired values for which the mean of the value falls between
50 and 300 colonies, d is the
Y.!*
dilution factor for the dilution taken into account
N C o r N T : l o g r o [ { 1 0 .( y + y ' ) } 1 2 . d l
yl,rere y andy'are paired values for which the mean of the value falls between 50 and 300 colonies,
d is the
dilution factor for the dilution taken into account
 MOF, Alm.del - 2015-16 - Bilag 660: Henvendelse af 27/9-16 fra Kenneth Riis, Lifeguarders, om MRSA - Gør man overhovedet noget ved problemet
1669735_0007.png
Description: Testing the fficacy of chemical disinfectantsand antiseptics
5
4
SampleID : 209 -209 / 2012
RepNo:111
DETOXY COLOR
name:
Sample
by
Sampled: client
Biskupiceu Lr-ilradovice
point: ROKOSPOLa.s.,
Sampling
Praha1
a.s.,Krakovsk| 1346115,
Client:ROKOSPOL
Samplingdate:20.9.2012
20.9.2012
delivered:
Sample
22.10.-10.12.2012
Testingdate:
0.5
amount: kg + 0.5kg
Delivered
sample
laboratory
BatchNo: 2700410,
Page:7
C.3 Evaluation of bactericidal and fungicidal activity of the product DETOXY COLOR on carriers
Tab No. 3.1 The efficacy of chemical disinfectant DETOXY
on cafflers
Bactericida and fungicidal activity ofthe product on caffi
Product test
Contact
Test
Strain
concentrations
tlme
temperature
lo/n1
locl
lminl
r00
2 hours
23
Pseudomonasaerusinosa ATCC 15442
t00
2 hours
z3
A.tneloillus niser ATCC 16 404
100
24 hours
ZJ
15442
Pseudomonasaerusinosa ATCC
COLOR on test strains - bactericidal and
Interfering
-
substances
conditions
lgR
CSN EN
lgR
r3697
>4
<4
niser ATCC 16 404
Aspersillus
Pseudomonasaerusinosa ATCC 15442
Asoerpillus niEer NICC 16 404
Pseudomonasaeruginosa ATCC 15442
Asne,rsillusniser ATCC 16 404
Asneroillus niser NICC 16 404
L)
L)
L)
LJ
24 hours
I week
I week
00
00
>3
>+
>3
>4
<3
>4
<3
00
00
00
ZJ
z-)
2 weeks
2 weeks
1 month
>3
>4
> t
>3
>3
>3
00
>3
Mikrobicidal effect of ME : Nc - Nd
Preparedby:
Hana Konevalikov5, Lab Technician
 MOF, Alm.del - 2015-16 - Bilag 660: Henvendelse af 27/9-16 fra Kenneth Riis, Lifeguarders, om MRSA - Gør man overhovedet noget ved problemet
1669735_0008.png
Description: Testing the fficacy of chemical disinfectantsand antiseptics
Sample : 2094-209 12012
ID
5
Rep
No: 111
Sample
name:DETOXY COLOR
Sampled: client
by
Sampling
point:ROKOSPOL Biskupice Luhadovice
a.s.,
u
Clienf ROKOSPOL Krakovsk|1346115,
a.s.,
Praha
I
Interpretation:
Results of tests are in Tabs.
Samplingdale:20.9.2012
Sample
delivered:
20.9.2012
Testingdate: 10.-10.12.2012
22.
Deliveredamount: kg + 0.5kg
0.5
BatchNo: 2700410,
laboratory
sample
Pase:
8
The tested concenhatedproduct DETOXY COLOR, batch No. 2700410, in the contacttime24 hours, under no
interfering substances,at temperature 23 "C + 2 "C, by *re dilution neutralization method, decreased on
carriersx feated by the product according to the manufacturer's requirements the number of alive microbes
Pseudomonqsaeruginosa ATCC l5442by 4 (lg) orders (EN 13697).
The tested concentratedproduct DETOXY COLOR, batch No. 2700410, in contact times 1 week, 2weeks and
I month, under no interfering substances,at temperature 23 "C + 2 "C, by the dilution neulu:alization
method,
decreased on carriers* treated by the product according to the manufacturer'srequirements the number of alive
microbes Aspergillus niger ATCC 16 404 by a (lg) orders (EN 13697).
x The test was modified according to the granted
testing scopeflexibility type I.
Conclusion:
The product DETOXY COLOR is capable of reducing the number of viable Pseudomonas aeruginosa cells
under defined conditions to the declaredvalues.
The productDEToxY col,oR is capable reducingthdnuinhbr.of..viable
of
Aspersillus
niger cells under
'
defined
conditions thedeclared
.,; ,1v,'.1--J:-*\f;t.1
to
values.
""""..
24.r.2ot3,Hodonin
#;i''/ "'o',.,59,,,#
\?
,'li*ryl1:
Ht
f;/ t";n*
-"'